Cigarette smoking impairs sperm bioenergetics.
نویسندگان
چکیده
OBJECTIVE The growing consensus on the negative impact of cigarette smoking on fertility prompted us to compare the rate of sperm respiration in smokers and non-smokers. MATERIALS AND METHODS Semen samples from 20 smokers and 58 non-smokers consulting at the andrology laboratory for fertility evaluation were used. Smoking was defined as consumption of at least a half a pack per day. A phosphorescence analyzer that measures O(2) concentration in sperm suspensions as function of time was used to determine the rate of respiration. In a sealed vial, the rate of sperm respiration (k) was defined as -d[O(2)]/dt; where [O(2)] was obtained from the phosphorescence decay rate of a palladium phosphor. [O(2)] in solutions containing sperm and glucose declined linearly with time, showing the kinetics of O(2) consumption was zero-order. Inhibition of O(2) consumption by cyanide confirmed the oxidations that occurred in the sperm mitochondrial respiratory chain. RESULTS There were no differences (p > 0.28) between smokers and non-smokers for ejaculate volume, motility, concentration, normal morphology, viability and hypo-osmotic swelling test. The rate (mean + or - SD, in microM O(2)/min/10(8) sperm) of sperm mitochondrial O(2) consumption in the smokers was 0.96 + or - 0.58 and in the non-smokers 1.39 + or - 0.67 (p = 0.004). CONCLUSIONS The rate of sperm respiration was significantly lower in smokers. This negative impact of cigarette smoking on sperm aerobic metabolism may, in part, explain the lower rate of fertility in smokers.
منابع مشابه
The Effect of Cigarette Smoking on Human Sperm Creatine Kinase Activity: As An ATP Buffering System in Sperm
متن کامل
Is smoking a risk factor for decreased semen quality? A cross-sectional analysis.
BACKGROUND Previous studies suggest a deleterious effect of cigarette smoking on semen quality, but their results have not been consistent. We studied the association between current smoking and semen characteristics and hormonal levels in a large group of healthy men. METHODS From 1987 to 2004, seven separate occupational or environmental semen quality studies were co-ordinated by our depart...
متن کاملP-6: The Impact of Cigarette Smoking on Semen Quality in Infertile Couples
Background: Approximately, one third of world's population older than 15 years of age smokes. Cigarette smoke contains toxic chemical, mutagenic and carcinogenic compounds. Some of these substances were found in seminal plasma.Over the past two decades, there has been an increasing body of evidence that cigarette smoking may impair human semen quality.The results of these studies are confl...
متن کاملCorrelation between Sperm Parameters and Sperm DNA Fragmentation in Fertile and Infertile Cigarette Smoking Patients
The present study to investigate that the correlation between sperm parameters and sperm DNA fragmentation in fertile and infertile cigarette smoking patient. Forty men undergoing infertility evaluation were divided into four groups according their smoking ability (A: 2 days ( n=10), B: 4 days ( n=10), C: 8 days ( n=10), D: 15 days ( n=10)) sperm parameters ( sperm concentration, pH, Viscosity,...
متن کاملEffect of Cigarette Smoking on Sperm Parameters in Men With Idiopathic Infertility
Background: Several studies have reported that cigarette smoking affects sperm parameters, especially semen quality, but their results are contradictory. Objective: This retrospective study aims to evaluate the relationship of sperm parameters with smoking in men with idiopathic infertility. Materials and Methods: This retrospective study was conducted on 1744 infertile men with at least on...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- International braz j urol : official journal of the Brazilian Society of Urology
دوره 36 1 شماره
صفحات -
تاریخ انتشار 2010